e c2 confocal laser scanning microscope  (Nikon)

Journal: Oncotarget

Article Title: Drug-induced ciliogenesis in pancreatic cancer cells is facilitated by the secreted ATP-purinergic receptor signaling pathway

doi: 10.18632/oncotarget.23335

Figure Lengend Snippet: ( A ) Quantification of the effect of apyrase treatment on ciliogenesis. ( B ) Representative images showing the effect of apyrase on ciliogenesis of cells exposed to the indicated drugs. Nuclei were stained with DAPI (blue) and cilia with an antibody against the cilium marker acetylated tubulin (green). All images were captured using Nikon C2 Eclipse Ni-E confocal microscope using a 60× objective lens. Data are presented as mean ± SEM, * p ≤ 0.05, ** p ≤ 0.005, *** p ≤ 0.0005. ( C ) Representative images showing the staining of cilia with an antibody against IFT88 (red), an alternative marker of the cilium.

Article Snippet: Images of primary cilia were captured by acquiring Z-stacks using Olympus FluoView-FV 1000 or Nikon C2 Eclipse Ni-E confocal laser scanning microscope using 60X oil immersion lens.

Techniques: Staining, Marker, Microscopy

Journal: Oncotarget

Article Title: Drug-induced ciliogenesis in pancreatic cancer cells is facilitated by the secreted ATP-purinergic receptor signaling pathway

doi: 10.18632/oncotarget.23335

Figure Lengend Snippet: ( A ) Quantification of extracellular ATP in cells treated with pannexin channel blocker (mefloquine), vesicular transport inhibitor (latrunculin B). ( B ) Quantification of the effect of mefloquine on ciliogenesis in CFPAC-1 cells. ( C ) Representative images showing the effect of pannexin channel blockade on ciliogenesis in CFPAC-1 cells exposed to a selection of ciliogenic drugs. Nuclei were stained with DAPI (blue) and cilia with an antibody against the cilium marker acetylated tubulin (green). All images were captured using Nikon C2 Eclipse Ni-E confocal microscope using a 60× objective lens. Data are presented as mean ± SEM, * p ≤ 0.05, ** p ≤ 0.005, *** p ≤ 0.0005.

Article Snippet: Images of primary cilia were captured by acquiring Z-stacks using Olympus FluoView-FV 1000 or Nikon C2 Eclipse Ni-E confocal laser scanning microscope using 60X oil immersion lens.

Techniques: Selection, Staining, Marker, Microscopy

Journal: Oncotarget

Article Title: Drug-induced ciliogenesis in pancreatic cancer cells is facilitated by the secreted ATP-purinergic receptor signaling pathway

doi: 10.18632/oncotarget.23335

Figure Lengend Snippet: ( A ) Effect of suramin, a blocker of P2 purinergic receptors on ciliogenesis in CFPAC-1 cells exposed to ciliogenic drugs. ( B ) Representative images of CFPAC-1 cells showing the effect of suramin on ciliation in untreated and treated cells. Nuclei were stained with DAPI (blue) and cilia with an antibody against the cilium marker acetylated tubulin (green). All images were captured using Nikon C2 Eclipse Ni-E confocal microscope using a 60× objective lens. Data are presented as mean ± SEM, * p ≤ 0.05, ** p ≤ 0.005, *** p ≤ 0.0005.

Article Snippet: Images of primary cilia were captured by acquiring Z-stacks using Olympus FluoView-FV 1000 or Nikon C2 Eclipse Ni-E confocal laser scanning microscope using 60X oil immersion lens.

Techniques: Staining, Marker, Microscopy